WebThe polymerase (catalog no. BIO-21105, Bioline, London, UK) was RNA was then converted into the first-strand cDNA using an used for the second-round amplification. The cycling conditions oligo-dT primer and a Tetro cDNA synthesis kit (catalog no. BIO- were initial denaturation at 95°C for 3 min, followed by 40 cycles 65043, Bioline, London ... WebMar 31, 2024 · A Tetro cDNA synthesis kit (catalogue no. BIO-65043, Bioline, London, UK) was used to synthesise cDNA using random primers, while following the manufacturer’s instructions. Two genes from each condition (YPD-28 °C, YPD-16 °C, and YP + glycerol-16 °C) showing significant transcriptional changes in RNAseq dataset were picked for further ...
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WebAug 5, 2016 · Bioline's Tetro cDNA synthesis kit was used to synthesis first strand cDNA using Salmonella enterica total RNA to quantify gene expression. A total of 1 microgram RNA was used and at the end of the … WebSnippet: RT-PCR analysis of selected genes RNA extraction was performed using a Qiagen RNeasy mini kit with QIAshredder and cDNA was synthesized using a Bioline Tetro … how many band members are in slipknot
Tetro™ cDNA Synthesis Kit - thomassci.com
WebThe Tetro cDNA Synthesis Kit is optimized for RT reactions using a wide range of total RNA amounts (100pg-2_g), such that long and low abundance cDNAs can be detected … WebJan 31, 2013 · cDNA synthesis was performed with Tetro Reverse Trancriptase kit (Bioline Ltd.) according to the manufacturer’s recommendation. Quantitative PCR was performed using real-time PCR (ABI PRISM 7900, Applied Biosystems). ... reverse transcription reaction using the designed miRNA specific stem loop-RT primer and … WebTetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10 pg-5 μg), such that long and low-abundance cDNAs can be detected … The Tetro cDNA Synthesis Kit is the more flexible solution. You selects the desired … High quality RNA. RNA was isolated from HeLa cells using ISOLATE II RNA Mini … Reproducible results. pUC19 plasmid was isolated from 5 mL E. coli overnight LB … Customer Service 5171 Wilfong Road Memphis, Tennessee 38134-5611 USA … The Tetro cDNA Synthesis Kit is the more flexible solution. You selects the desired … Product Description. By combining the stringency of CTAB lysis with the speed … The MyTaq Extract-PCR Kit and a corresponding kit from Supplier S were … Fig. 1 Highly successful genotyping of a Class 4 (A/T) SNP using SensiFAST … A 340 bp (A) and a 450 bp (B) fragment of the myc gene, a 525 bp (C) fragment of … Toggle Nav Bioline’s new logo, ... Tetro cDNA Synthesis Kit; Molecular Biology … how many band members in slipknot