Cyanine dyes are used as fluorescent markers in oligonucleotide synthesis, (2) primarily for molecular diagnostics such as the preparation of probes used in monitoring real-time PCR, fluorescence in situ hybridisation (FISH) and in Surface-Enhanced Resonance Raman Spectroscopy (SERRS) based DNA … See more Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (LK4050) with the exception of LK2521where anhydrous alcohol free DCM is required. Adjust accordingly for other concentrations. For µmol pack sizes, … See more These products are light-sensitive and are susceptible to oxidation when left exposed to air and/or moisture. Store dry in a freezer, in a sealed dark or amber container at –20°C. Similarly, modified oligonucleotides … See more A 10min coupling time is recommended for all phosphoramidites. Due to the milder conditions required when deprotecting cyanine-labelled … See more As these reagents require no deprotection themselves, the oligonucleotide deprotection strategy employed is guided by the protective groups on the rest of the oligo. However, the mildest possible conditions are … See more WebEF5 Hypoxia Detection Kit, Cyanine 3 MSDS (material safety data sheet) or SDS, CoA …
荧光染料FITC、Alexa Flour、Cyanine介绍 - 知乎 - 知乎专栏
WebApr 12, 2024 · Sodium cyanide is a highly toxic and water-soluble inorganic compound with the chemical formula NaCN. It is a white solid that is used mainly in the mining industry to extract gold and other ... WebCyanine 3 and Cyanine 5 supports are also offered to allow simpler production of 3’ cyanine dye-labelled oligonucleotides. Deprotection of oligos containing Cyanine dyes may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. If there is a need to use ammonium hydroxide … can colon cancer cause kidney failure
BLACK HOLE QUENCHER™ (BHQ™) - Sigma-Aldrich
WebCompare all formats See Cyanine3 spectral data Description This polyclonal goat anti … WebJan 6, 2024 · 别称:脂溶性CY3-COOH,CY3标记羧酸,,Cy3 Carboxylic acids,Cyanine3-COOH,CY3羧基. CAS:1032678-01-5(氯化). 外观/Appearance:红色固体/Red Solid. 分子式/Molecular formula:C30H37ClN2O2. 分子量/Molecular weight:493.08. 纯度:95%+. 溶解性:溶于有机溶剂(DMF、DMSO、二氯甲烷),难溶于水. 储存 ... Web1. Mixture A: in an 8 μL reaction volume, mix 2 μL D-loop reaction buffer (5 × stock), 4 μL double-distilled water, 1 μL of Dmc1 (30 μM stock), and 1 μL of Cy5-ssDNA (300 nM stock). Incubate the mixture in a water bath at 37°C for 5 min.. 2. Mixture B: mix 1 μL Hop2-Mnd1 (5 μM stock) with 1 μL plasmid pRS306 (50 nM stock), incubate at room temperature for 3 … can colon cancer spread to cervix