WebThe Bradford is recommended for general use, especially for determining protein content of cell fractions and assesing protein concentrations for gel electrophoresis. Assay materials including color reagent, protein standard, and instruction booklet are available from Bio-Rad Corporation. The method described below is for a 100 µl sample ... WebSep 14, 2024 · The BCA assay revealed that all cell extracts had a similar mean protein content around 15 mg/mL. In contrast to the shaking flask extracts, the deviations between the biological replicates were higher than the deviations between the different lysis settings within a single replicate (Figure S4). ... This work was funded by the Deutsche ...

BCA Assay Thermo Fisher Scientific - US

WebThe key step is immobilization of the antigen of interest, accomplished by either direct adsorption to the assay plate or indirectly via a capture antibody that has been attached to the plate. The antigen is then detected either directly (labeled primary antibody) or indirectly (such as labeled secondary antibody). WebModule and the BCA Protein Assay is 5 µg/mL (Figure 1) and the maximum is 2 mg/mL (Figure 2). The Enhanced Protocol requires a 60°C incubation for 30 minutes. The Standard Protocol permits either a 30-minute incubation at 37°C or a 2 hour-incubation at room temperature. Figure 1. BCA Enhanced Protocol assay was performed on the Modulus … chinese crownhill

BCA Colorimetric Protein Assay - Protocol - OneLab

WebBCA is a weak acid composed of two carboxylated quinoline rings and serves the purpose of the Folin reagent in the Lowry assay, namely, to react with the product (Cu1 + ions) of complexes between copper ions and peptide bonds to produce a purple end product that strongly absorbs at 562 nm ( Smith et al., 1985 ). WebMar 8, 2016 · The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu 2+ ions, in a temperature dependent reaction, from the copper WebThe BCA protein assay is used for quantitation of total protein in a sample. The principle of this method is that proteins can reduce Cu+2 to Cu+1 in an alkaline solution (the biuret … chinese crotch rocket